ABSTRACT
This chapter discusses the selective movement of proteins throughout the cell, starting from the endoplasmic reticulum (ER). It begins with the visualization of the movement of translated proteins through the ER by electron microscopy, followed by evidence for the signal hypothesis. Next, the chapter discusses experiments using yeast which led to the discovery of many different proteins involved in protein transport throughout the cell. Finally, we look at the mechanism of protein transport from the ER, to the Golgi apparatus, and finally, to the membrane, using an intricate array of proteins that allow the budding-off and fusion of cargo-containing vesicles with membranes, which ensure that vesicles are targeted to the correct cellular structure.
