ABSTRACT

With a history that likely dates back to the dawn of human civilization more than 10,000 years ago, and a record that includes the domestication and selective breeding of plants and animals, the harnessing of fermentation process for bread, cheese, and brewage production, and the development of vaccines against infectious diseases, biotechnology has acquired a molecular focus during the 20th century, particularly following the resolution of DNA double helix in 1953, and the publication of DNA cloning protocol in 1973, and transformed our concepts and practices in disease diagnosis, treatment and prevention, pharmaceutical and industrial manufacturing, animal and plant industry, and food processing.

While molecular biotechnology offers unlimited opportunities for improving human health and well-being, animal welfare, agricultural innovation and environmental conservation, a dearth of high quality books that have the clarity of laboratory manuals without distractive procedural details and the thoroughness of well-conversed textbooks appears to dampen the enthusiasm of aspiring students. In attempt to fill this glaring gap, Handbook of Molecular Biotechnology includes four sections, with the first three presenting in-depth coverage on DNA, RNA and protein technologies, and the fourth highlighting their utility in biotechnology. Recognizing the importance of logical reasoning and experimental verification over direct observation and simple description in biotechnological research and development, the Introduction provides pertinent discussions on key strategies (i.e., be first, be better, and be different), effective thinking (lateral, parallel, causal, reverse, and random), and experimental execution, which have proven invaluable in helping advance research projects, evaluate and prepare research reports, and enhance other scientific endeavors.

Key features

  • Presents state-of-the-art reviews on DNA, RNA and protein technologies and their biotechnological applications
  • Discusses key strategies, effective thinking, and experimental execution for scientific research and development
  • Fills the gap left by detailed-ridden laboratory manuals and insight-lacking standard textbooks
  • Includes expert contributions from international scientists at the forefront of molecular biotechnology research and development

Written by international scientists at the forefront of molecular biotechnology research and development, chapters in this volume cover the histories, principles, and applications of individual techniques/technologies, and constitute stand-alone, yet interlinked lectures that strive to educate as well as to entertain. Besides providing an informative textbook for tertiary students in molecular biotechnology and related fields, this volume serves as an indispensable roadmap for novice scientists in their efforts to acquire innovative skills and establish solid track records in molecular biotechnology, and offers a contemporary reference for scholars, educators, and policymakers wishing to keep in touch with recent developments in molecular biotechnology.

chapter 1|16 pages

Molecular Biotechnology

From Past Achievements to Future Breakthroughs

part Section I|98 pages

DNA Technologies

chapter 182|7 pages

DNA Structure and Function

chapter 3|8 pages

DNA Purification

chapter 4|9 pages

DNA Amplification

chapter 5|8 pages

DNA Sequencing

chapter 8|8 pages

DNA Mutagenesis

chapter 9|11 pages

CRISPR/Cas9-Mediated Genome Editing

Current Status

part Section II|78 pages

RNA Technologies

chapter 11611|7 pages

RNA Structure and Function

chapter 13|6 pages

RNA Amplification

chapter 15|7 pages

RNA Sequencing

chapter 16|4 pages

RNA Cloning

part Section III|138 pages

Protein Technologies

chapter 19419|7 pages

Protein Structure and Function

chapter 21|5 pages

Protein Sequencing

chapter 24|9 pages

Polyclonal Antibodies

chapter 26|13 pages

Recombinant Antibodies

chapter 27|8 pages

Protein Modification

chapter 28|6 pages

Protein-Protein Interaction

chapter 29|13 pages

Protein-DNA Interactions

A Technical Overview

chapter 30|6 pages

Protein-RNA Interaction

chapter 31|5 pages

Protein-Lipid Interaction

chapter 32|8 pages

Protein-Carbohydrate Interaction

part Section IV|152 pages

Molecular Approaches to Biotechnology

chapter 33233|8 pages

Molecular Systematics

chapter 35|5 pages

Immunodiagnostics

chapter 36|10 pages

Mutation Detection

chapter 38|15 pages

Antimicrobial Resistance

Causes, Mechanisms, Origins and Characterization

chapter 41|20 pages

Genomics

chapter 42|5 pages

Transcriptomics

chapter 43|14 pages

Proteomics

chapter 45|10 pages

Biosensors

part |224 pages

Therapeutics

chapter 48446|8 pages

Probiotics and Prebiotics

chapter 47|10 pages

Dietary Supplements

chapter 49|19 pages

Phage Therapy

A Clinician's Guide

chapter 50|9 pages

Gene Therapy

chapter 54|5 pages

Miscellaneous Applications

Forward and Reverse Genetics

chapter 61|14 pages

Transgenic Plants

chapter 62|12 pages

Bioinformatics